- Title
- Novel Bacillus cereus strain from electrokinetically remediated saline soil towards the remediation of crude oil
- Creator
- Gao, Yong-chao; Guo, Shu-hai; Wang, Jia-ning; Zhang, Wen; Chen, Guan-hong; Wang, Hui; Du, Jianhua; Liu, Yanju; Naidu, Ravi
- Relation
- Environmental Science and Pollution Research Vol. 25, Issue 26, p. 26351-26360
- Publisher Link
- http://dx.doi.org/10.1007/s11356-018-2495-z
- Publisher
- Springer
- Resource Type
- journal article
- Date
- 2018
- Description
- A new strain SWH-15 was successfully isolated after initial electrokinetic remediation experiment using the same saline soil sampled from Shengli Oilfield, China. Four methods (morphological and biochemical characteristics, whole-cell fatty acid methyl esters (FAMEs) analysis, 16S rRNA sequence analysis and DNA G + C content and DNA–DNA hybridization analysis) were used to identify the taxonomic status of SWH-15 and confirmed that SWH-15 was a novel species of the Bacillus (B.) cereus group. Then, we assessed the degrading ability of the novel strain SWH-15 to crude oil through a microcosm experiment with four treatments, including control (CK), bioremediation using SWH-15 (Bio), electrokinetic remediation (EK), and combined bioremediation and electrokinetic remediation (Bio + EK). The results showed that the Bio + EK combined remediation treatment was more effective than the CK, Bio, and EK treatments in degrading crude oil contaminants. Bioaugmentation, by addition of the strain SWH-15 had synergistic effect with EK in Bio + EK treatment. Bacterial community analysis showed that electrokinetic remediation alone significantly altered the bacterial community of the saline soil. The addition of the strain SWH-15 alone had a weak effect on the bacterial community. However, the strain SWH-15 boosted the growth of other bacterial species in the metabolic network and weakened the impact of electrical field on the whole bacterial community structure in the Bio + EK treatment.
- Subject
- soil; crude oil; electrokinetic remediation; whole-cell fatty acid methyl esters (FAMEs); illumina sequencing; SDG 15; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1448089
- Identifier
- uon:43309
- Identifier
- ISSN:0944-1344
- Language
- eng
- Reviewed
- Hits: 1483
- Visitors: 1483
- Downloads: 0